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One-year-old fish were histologically sexed.

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Lysate was analyzed for Luc activity using the luciferase assay system Promega in an Orion Microplate luminometer Berthold.

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This, as statistical practice dictates, could be only done for those patterns in which the expected frequency for a given treatment was 5 or higher, which, with four groups means whose observed frequency was 20 or higher.

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Description: In the present study, the lack of effects of estrogen treatment on gonadal sb cyp19a promoter methylation levels supports the idea that increased methylation is the cause of lower cyp19a expression and not the other way around. Gorelick R Evolution of dioecy and sex chromosomes via methylation driving Muller's ratchet.

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